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Connectomics deep drive into challenge project

Methodology to analyse calcium fluorescence data

One of the great challenges in modern science is to understand the structure of the human brain. Neurologists want to work out the complete map of connections between neurons, it’s a wiring diagram and a structure known as the human connectome. If we predict the connection by the firing of the Calcium ion we can predict the connections between neurons using calcium fluorescence data. first, so we Are moving to analyses the “small” dataset involving a network of 100 neurons. 

The process must go through deep learning. We initially processed several steps, first we include clipping maximum values to reduce noise and extending each series values to maximize contrast. These steps will help to recognize neurons visually but it will not improve performance Finally, the training datasets have semantic differences we must know the process like labeling technique etc. In some cases, we may improve performance by training one model per dataset.



Here we analysis several methods that can be applied to calcium imaging data, without the direct need for converting the data to spike trains which is the more traditional and popular way of connectivity analysis.  We can apply generalized different sets of calcium imaging data given, and infer the directed functional connectivity network, in which a directed edge implies a direct causal influence by source neuron to sink neuron. The transfer influence measure is time-dependent but requires no prior statistical assumptions on neuron firing patterns and network topology, hence model-free and applicable in face of challenges. We will further talk about in the next post.

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